CRISPR/Cas9 plasmid editing question
May 11, 2013 7:41 PM Subscribe
Hello all, I would like to try to use the exciting new CRISPR/Cas9 system two make 2 ds breaks across about 1kb, then via homologous recombination introduce a much smaller selection marker. This done within the LB / RB of the Ti-plasmid of Agrobacterium tumefaciens, sequenced of course. I understand requirements of G-N20x-GG sequence, so assuming that is met. Thanks everyone!
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This reads like a hilarious plot to a tom cruise movie. In the end we all get investigated by the FBI.
Somehow i think if you have access to the equipment you should have access to the training, or the people who were trained to use it.
posted by hobo gitano de queretaro at 8:51 PM on May 11, 2013
Somehow i think if you have access to the equipment you should have access to the training, or the people who were trained to use it.
posted by hobo gitano de queretaro at 8:51 PM on May 11, 2013
I have nothing to add, except that this is the best statement/non question on MeFi. Happy to hook you up with my bio/engineering friends who mig have answers, of course. But I like the mystery.
posted by blahblahblah at 8:53 PM on May 11, 2013
posted by blahblahblah at 8:53 PM on May 11, 2013
This thread is closed to new comments.
posted by Tandem Affinity at 8:33 PM on May 11, 2013