Chromatography for newbies
July 19, 2014 2:39 PM Subscribe
Chemists! I have a question about chromatography. Specifically, what is the best way to isolate a specific molecule, if I want to produce large volumes of this isolated molecule?
posted by special agent conrad uno to Technology (7 answers total)
So I will have a mixture which will be about 87% of this one specific molecule. I would like to refine it to over 99% purity. I will be refining about 1 kg of this per day (or 380 kg per year), which should be at an initial volume of around 1 liter, so similar density to water.
I feel like HPLC is the way to go, but I have very little experience with it, and I don't know what kind of volume HPLC can do. I've heard about countercurrent chromatography as well, but I really don't know anything about that.
Level of experience: took some chem in college, remember a decent amount of it.