Whole Genome Sequencing with Single Base Extension (Ilumina Ifinium)
July 10, 2020 1:47 AM Subscribe
I'm trying to understand how this exactly works but I don't get it. According to this figure every SNP gets one bead in the array, the DNA hybridizes with it and you get a red signal if it's AA, yellow AB and green if it's BB. But how do they assign the correct Nucleotide? If the two oligos on the bead end in G and T, you can distinguish CC, AA, AC. But if there is no signal for that bead, it can be either G ,T or GT? What am I missing, I have a feeling it's quite obvious... Paper is here.