Dear MetaFilter, I am new to this forum and this is my first post, hi!
I am in a bit of a pickle about a work project, and hope that I may be able to get some help by some fellow sciencey people. The science forums are rarely read and badly out of date, or I would have posted at one of those.
I have a deadline for a miRNA PCR experiment, and I have already run the cDNA and PCR for the miRNA. All I have to do is run the miRNA on a gel.
We don't have acrylamide! And neither does our neighboring lab. We only have Agarose with which to make gels, which is only for large fragments, not small miRNA fragments of 20-40 bp.
As the percentage of agarose in the gel goes higher, the smaller the size of the base pairs are that can be measured. Could I increase this percentage enough to work for miRNA?
Out of respect for the community that doesn't like line returns, I have entered the specifics of this post in the Extended Explanation box, because to me, this post reads like a jumble of nonsense without some kind of organization. [more inside]
I'm looking for good free software tools for doing basic molecular biology work - sequence alignments, DNA-protein translation, restriction mapping, and PCR primer design. Got any recommendations? [more inside]
Molecular Biology / PCR Gel Electrophoresis Help? Help in elucidation of source of spurious banding? [more inside]
Can anyone recommend a compact benchtop centrifuge that accepts 96-well plates? [more inside]
Calling all biologists - I've amplified a 700 bp region of cDNA for the gene PGI and now am trying to amplify this region using genomic DNA. Way more detail inside... [more inside]
Is it a good idea at the moment to have a viral PCR test for HIV 10 days after being exposed to risk? [more inside]