TMB Substrate Creation
July 6, 2006 6:52 PM Subscribe
Biology Research Filter: How do you make your own TMB substrate solution for use with an ELISA kit?
posted by sicem07 to Science & Nature (4 answers total) 1 user marked this as a favorite
My research group is running immunassays for used detection of aldosterone. We're tired of buying the plates ($250 a pop), so we're looking at producing our own. The TMB substrate for use with the HRP-conjugate is part of the reaction that is included with all of the purchased plates, but we're having trouble creating our own. The companies don't give out any concentrations or directions for creating solutions, since they want you to purchase their own. We've found several protocols that involve a citric acid buffered solution, but our TMB won't dissolve. It will dissolve in DMSO, but our results aren't great. I understand that this is a little specific, but I'm sure someone out there in the vast world of MetaFi has experience with this. Thanks for the help.