February 15, 2006 8:32 AM Subscribe
BioChemistryFilter: How do I read the vMax and Km off of a Lineweaver-burk plot?
posted by tnai to Science & Nature (9 answers total) 1 user marked this as a favorite
This is probably so easy as to be obvious but having sat here all afternoon with lecture notes and biochem books i'm still none the wiser so... say i have a Lineweaver-burk plot that looks similar to this:
Assuming there are values along the x & y axis, how do i read the vMax and Km off of this plot. Is it as simple as Km being the "up" measurement on the "slope" and vMax being the "across" measurement (or vice-versa)? Or is there something more complex involved to get these values?
Also, if it is more complex, are there any websites available that can explain the Lineweaver-burk plot in simple, baby-like, steps?
Thanks for any help!