DNA extraction from sperm
May 6, 2005 10:52 AM Subscribe
Does anyone have a good, detailed protocol for nuclear DNA extraction from a human (or other mammalian) sperm sample?
posted by nprigoda to science & nature (5 answers total)
Our lab will be receiving a sperm sample and need to get DNA to use in PCR (and maybe quantitative PCR). Our problem is, we've never received semen before. I've worked with blood and tumor samples, but I'm concerned that there is enough difference between blood and semen that I can't use my normal protocol.
Also, I don't really know what to suggest to the lab sending the sample. Should we freeze the sample to avoid DNA degradation?